Socializing Kombucha Making as Science and Healthy Lifestyle Education at SMAN 1 Kota Serang
Main Article Content
Abstract
This Community Service (PkM) activity aimed to enhance high school students' science literacy and healthy lifestyle awareness through socialization and live demonstration of kombucha production as an application of conventional biotechnology. The main underlying problem was the students' limited hands-on learning experience regarding fermentation, as biology learning at school had predominantly been one-way and theoretical. The activity was conducted on October 9, 2025, at SMAN 1 Kota Serang, involving 80 tenth-grade students from class X-7. The implementation method combined community education through interactive lectures and practical training through live kombucha-making demonstrations. The program evaluation was conducted qualitatively and descriptively through direct observation of the activity flow and documentation of student responsiveness during the lecture and practical sessions. The evaluation results indicated that the activity was executed interactively, with high student participation and enthusiasm throughout the session. During the lecture, students actively participated in question-and-answer discussions regarding the function of Symbiotic Culture of Bacteria and Yeast (SCOBY) and the safety of fermented beverages. During the practical demonstration, students carefully observed each procedural step, ranging from container sterilization and sweet tea substrate preparation to starter culture inoculation. Through this direct observation, students gained concrete insights into the connection between biological concepts and practical biotechnology in daily life. Overall, this activity proved effective as a contextual learning tool to introduce applied science applications while promoting healthy lifestyles based on functional foods in secondary schools.
Article Details

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
References
Agustin, R. D., Giriwono, P. E., & Prangdimurti, E. (2024). Variasi lama waktu fermentasi terhadap karakteristik kimia teh kombucha : meta-analisis. Agrointek, 18(3), 538–551. https://doi.org/10.21107/agrointek.v18i3.19200
Alkan, G., Erol, N. M. K., Yıkmış, S., Er, H., Öğüt, S., & Yinanç, A. (2025). Kombucha’s functional features and fermentation dynamics: a bibliometric assessment in sustainable food production. Frontiers in Sustainable Food System, 9, 1593348. https://doi.org/10.3389/fsufs.2025.1593348
Ariestanti, C. A., Pirena, K., & Nugroho, W. S. (2023). Pengajaran pembuatan minuman fermentasi berbahan dasar teh dan susu. Jurnal Pembelajaran Pemberdayaan Masyarakat (JP2M), 4(2), 575–584. https://doi.org/10.33474/jp2m.v4i2.20767
Azizah AN, Darma GC, Darusman F. (2020). Formulasi SCOBY (Symbiotic Culture of Bacteria and Yeast) dari Raw Kombucha Berdasarkan Perbandingan Media Pertumbuhan Larutan Gula dan Larutan Teh Gula. Prosiding Farmasi
Chahya, A., Sagurung, M. D., Situmorang, R., & Advinda, L. (2025). Pembuatan Teh Kombucha Berbahan Dasar Teh Hijau. Biogenerasi, 10(2), 1271–1276. https://doi.org/https://doi.org/10.30605/biogenerasi.v10i2.5766
Chandra, M. A., Marliadi, R., & Wahyudi. (2023). Pelatihan Pembuatan Teh Hitam Kombucha ( Rosa Felicia L .) Sebagai Eunoia Kesehatan dalam Menjaga Body Immunity pada Kelompok Wanita Tani Kurnia Lestari. I-Com: Indonesian Community Journal, 3(4), 1839–1847. https://doi.org/https://doi.org/10.33379/icom.v3i4.3494
Chong, A. Q., Lau, S. W., Chin, N. L., Talib, R. A., & Basha, R. K. (2023). Fermented Beverage Benefits: A Comprehensive Review and Comparison of Kombucha and Kefir Microbiome. Microorganisms, 11(5), 1344. https://doi.org/https://doi.org/10.3390/microorganisms11051344
Değirmencioğlu, N., Yıldız, E., Sahan, Y., Güldas, M., & Gürbüz, O. (2021). Impact of tea leaves types on antioxidant properties and bioaccessibility of kombucha. Journal of Food Science and Technology, 58(6), 2304-2312
Dewi AS, Atifah Y, Farma SA, Yuniarti E, Fadhilla R. (2021). Pentingnya Konsumsi Probiotik untuk Saluran Pencernaan dan Kaitannya dengan Sistem Kekebalan Tubuh Manusia. InProsiding Seminar Nasional Biologi
Faizah, Khairunnisa, A., Latifasari, N., & Kurniawati, A. D. (2024). Kombucha dan Sifat Fungsionalnya : Studi Pustaka. Jurnal Sains Dan Teknologi Pangan, 9(5), 7729–7741. https://doi.org/https://doi.org/10.63071/42pr8595
Fraiz, G. M., Bonifácio, D. B., Paulo, R. S. De, Teixeira, C. M., Stampini, H., Martino, D., Augusto, F., Barros, R. De, Milagro, F. I., & Bressan, J. (2025). Benefits of Kombucha Consumption: A Systematic Review of Clinical Trials Focused on Microbiota and Metabolic Health. Fermentation, 11(6), 353. https://doi.org/https://doi.org/10.3390/fermentation11060353
Hananta, L., Kurniawan, S. V., Lonah, Arieselia, Z., Surjono, E., Setiawan, J., Lieputra, A. A., Bunardi, D. P., Tjendratama, J. P., Satyadi, R. V., & Dewi, R. (2025). Antioxidant, Antimicrobial, Anti-Inflammatory, and Gut Microbiota Modulation Effects of Kombucha: a Literature Review. Journal of Urban Health Research, 3(2), 1–9. https://doi.org/https://doi.org/10.25170/juhr.v3i2.6445
Jakubczyk, K., Kupnicka, P., Melkis, K., Mielczarek, O., Walczyńska, J., Chlubek, D., & Janda-Milczarek, K. (2022). Effects of fermentation time and type of tea on the content of micronutrients in kombucha fermented tea. Nutrients, 14(22), 4828. https://doi.org/https://doi.org/10.3390/nu14224828
Jayabalan, R., Malini, K., Sathishkumar, M., Swaminathan, K., & Yun, S.E. 2010. Biochemical characteristics of tea fungus produced during kombucha fermentation. Food Science and Biotechnology, 19(3), 843-847
Khasanah, D. U., & Dewi, E. N. (2024). Karakteristik Produk Minuman Kombucha Berdasarkan Komposisi Bahan Baku dan Waktu Fermentasi. Distilat Jurnal Teknologi Separasi, 10(4), 754–763. https://doi.org/https://doi.org/10.33795/distilat.v10i4.6593
Laureys, D., Britton, S.J., & Clippeleer, J.D. 2020. Kombucha tea fermentation: a review. Journal of the American Society of Brewing Chemist, 78(3), 165-174. https://doi.org/10.1080/03610470.2020.1734150
Li, X., Tso, N., Huang, S., Wang, J., Zhou, Y., & Liu, R. (2025). A Comprehensive Evaluation of Microbial Synergistic Metabolic Mechanisms and Health Benefits in Kombucha Fermentation: A Review. Biology, 14(8), 952. https://doi.org/https://doi.org/10.3390/biology14080952
Liao, T., Li, X., Fan, L., Zhang, B., Zheng, W., Hua, J., Li, L., Mahror, N., & Cheng, L. (2024). Nature of back slopping kombucha fermentation process: insights from the microbial succession, metabolites composition changes and their correlations. Frontiers in Microbiology, 15, 1433127. https://doi.org/10.3389/fmicb.2024.1433127
Maghfirah, D., Pulungan, A. F., Ridwanto, & Yuniarti, R. (2025). Comparison of phenolic and antioxidant contents in tea brewing and kombucha tea variants by visible spectrophotometry. Journal of Pharmaceutical and Sciences, 8(3), 1384–1397. https://doi.org/https://doi.org/10.36490/journal-jps.com.v8i3.952
Motafeghi, F., Mortazavi, P., Shahsavari, R., Shaker, M., Mohammadi-Berenjestanaki, H., & Shokrzadeh, M. (2023). Kombucha mushroom extract: anticancer, antioxidant, and antimicrobial properties. Applied In Vitro Toxicology, 9(3), 90-103
Nisak, Y. K. (2023). Studi aktivitas antioksidan minuman fermentasi kombucha: kajian pustaka. AGRITEPA, 10(1), 23–34. https://doi.org/https://doi.org/10.37676/agritepa.v10i1.3311
Putri, D. A., Komalasari, H., Ulpiana, M., Salsabila, A., & Arianto, A. R. (2023). Produksi Kombucha Teh Hitam Menggunakan Jenis Pemanis dan Lama Fermentasi Berbeda. Jurnal Kolaboratif Sains, 6(7), 640–656. https://doi.org/https://doi.org/10.56338/jks.v6i7.3713
Rantisari, A. M. D., Sulfiani, S., Rosmayanti, V., & Saleh, A. (2023). Pemanfaatan teh kombucha untuk kesehatan dan kecantikan kulit pada masyarakat di Kabupaten Maros. Indonesia Berdaya, 4(4), 1277–1286. https://doi.org/https://doi.org/10.47679/ib.2023553
Salomon, L. L., Kristina, H. J., Aurelia, N., Sugianto, R., & Gunawan. (2024). Pelatihan Pembuatan Produk Minuman Probiotik Kombucha dan Spa Kombucha Rumahan. Jurnal Bakti Masyarakat Indonesia, 7(1), 117–126.
Saputro, A. E., Laswati, D. T., Puspitasari, D., & Laksana, E. M. B. (2025). Karakteristik Fisikokimia Dan Mikrobiologis Minuman Fermentasi Kombucha Kunir Putih (Curcuma Mangga Val.) Sebagai Pangan Fungsional. Jurnal Sains Dan Teknologi, 10(4), 8731–8742. https://doi.org/https://doi.org/10.63071/9znqwv53
Yunita, M., Warella, J. C., Astuty, E., Asmin, E., & Ohiwal, M. (2024). Pemberdayaan Masyarakat Melalui Edukasi Manfaat Probiotik dan Pelatihan Pembuatan Minuman Teh Kombucha. JMM: Jurnal Masyarakat Mandiri, 8(2), 1–4. https://doi.org/https://doi.org/10.31764/jmm.v8i2.21658