Formulation Of A Natural Mouth Spray Containing Clove Flower Extract (Syzygium aromaticum) And Peppermint Leaf Essential Oil (Mentha piperita L.) As A Mouth Freshener
Main Article Content
Abstract
Halitosis is an oral health problem that can reduce quality of life and self-confidence. The use of natural mouth sprays offers a promising alternative for maintaining oral freshness. Clove flower extract (Syzygium aromaticum) contains eugenol, which has antibacterial properties, while peppermint essential oil (Mentha piperita L.) contains menthol, which provides a refreshing sensation. This study aimed to formulate a mouth spray combining clove flower extract and peppermint essential oil and to evaluate its physical quality, stability, and panelist acceptance. This experimental study used four formulations: Blank Formulation (F0), Formulation 1 (F1), Formulation 2 (F2), and Formulation 3 (F3). The evaluation included organoleptic testing, pH measurement, spray pattern analysis, spray weight measurement, stability testing using a cycling test, and hedonic testing involving 31 panelists. Hedonic data were analyzed using the Friedman test, followed by pairwise Wilcoxon Signed-Rank tests with Bonferroni correction, while physical parameters were analyzed descriptively using mean ± standard deviation (SD). The results showed that all formulations met the evaluated physical quality parameters, with pH values ranging from 6.53 to 7.43. During the cycling test, the organoleptic properties remained unchanged, although a decrease in pH was observed, while the pH values remained within the acceptable range. The hedonic test revealed significant differences in panelists’ acceptance among specific formulation pairs, with Formula 1 showing the highest mean acceptance scores across the six sensory parameters. Based on the study results, the combination of clove flower extract and peppermint essential oil was successfully formulated into a mouth spray with good physical quality and stability and has the potential to be developed as a natural mouth freshener.
Article Details

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
References
Abdelrahman, S. M., M. El Samak, L. M. F. El-Baz, A. M. S. Hanora, P. Satyal, and N. S. Dosoky. 2024. “Effects of Mint Oils on the Human Oral Microbiome: A Pilot Study.” Microorganisms 12 (8). https://doi.org/10.3390/microorganisms12081538.
Akaji, E. A., N. Folaranmi, and O. Ashiwaju. 2014. “Halitosis: A Review of the Literature on Its Prevalence, Impact and Control.” Oral Health & Preventive Dentistry 12 (4): 297–304. https://doi.org/10.3290/j.ohpd.a33135.
Alshehri, F. A. 2016. “Knowledge and Attitude of Saudi Individuals toward Self-Perceived Halitosis.” Saudi Journal for Dental Research 7 (2): 91–95. https://doi.org/10.1016/j.sjdr.2015.11.003.
Anung Anindhita, M., N. Oktaviani, U. Pekalongan, J. Sriwijaya No., and K. Pekalongan Jawa Tengah. 2020. “Formulasi Spray Gel Ekstrak Daun Pandan Wangi Sebagai Antiseptik Tangan.” Metha Anung Anindhita dan Nila Oktaviani 9 (1). http://ejournal.poltektegal.ac.id/index.php/parape.
Dubar, M., A.-C. Decock, J. Petit, E. Delcourt-Debruyne, A. Blaizot, and K. Agossa. 2023. “How Effective Are Essential Oils and Essential Oil Constituents in the Management of Intra-Oral Halitosis: A Systematic Review.” Journal of Herbal Medicine 39: 100663. https://doi.org/10.1016/j.hermed.2023.100663.
Elbestawy, M. K. M., G. M. El-Sherbiny, and S. A. Moghannem. 2023. “Antibacterial, Antibiofilm and Anti-Inflammatory Activities of Eugenol Clove Essential Oil against Resistant Helicobacter pylori.” Molecules 28 (6). https://doi.org/10.3390/molecules28062448.
Farid, Am. 2021. “Formulasi dan Uji Stabilitas Pasta Gigi Cangkang Telur Ayam Ras (Gallus sp) dengan Variasi Konsentrasi Na.CMC.” Fito Medicine: Journal Pharmacy and Sciences 12. http://journal.unpacti.ac.id/index.php/fito.
Huda, M., R. Djayasinga, and D. S. Ningsih. 2018. “Efektivitas Ekstrak Bunga Cengkeh (Eugenia aromatica) terhadap Pertumbuhan Bakteri Staphylococcus aureus.” Jurnal Analis Kesehatan 7 (1): 710. https://doi.org/10.26630/jak.v7i1.934.
Kementerian Kesehatan RI. 2017. Profil Kesehatan Indonesia. Kementerian Kesehatan Republik Indonesia.
Kementerian Kesehatan RI. 2018. Laporan Nasional RISKESDAS 2018. Lembaga Penerbit Badan Penelitian Pengembangan Kesehatan. https://repository.kemkes.go.id/book/1323.
Kubala, E., P. Strzelecka, M. Grzegocka, D. Lietz-Kijak, H. Gronwald, P. Skomro, and E. Kijak. 2018. “A Review of Selected Studies That Determine the Physical and Chemical Properties of Saliva in the Field of Dental Treatment.” BioMed Research International 2018: 1–13. https://doi.org/10.1155/2018/6572381.
Lestari, E. D., and K. R. P. Sari. 2021. Penentuan Kadar Flavonoid Total Fraksi n-Heksan, Etil Asetat, dan Air dari Ekstrak Etanol Bunga Cengkeh (Syzygium aromaticum) dengan Metode Spektrofotometri UV-Vis. https://id/eprint/726.
Maggini, V., G. Semenzato, E. Gallo, A. Nunziata, R. Fani, and F. Firenzuoli. 2024. “Antimicrobial Activity of Syzygium aromaticum Essential Oil in Human Health Treatment.” Molecules 29 (5): 999. https://doi.org/10.3390/molecules29050999.
Moghtader, M. 2013. “In Vitro Antifungal Effects of the Essential Oil of Mentha piperita L. and Its Comparison with Synthetic Menthol on Aspergillus niger.” African Journal of Plant Science 7 (11): 521–527. https://doi.org/10.5897/ajps2013.1027.
Mudia Alti, R. 2023. “Formulation of Mouthwash with Clove and Peppermint Extract.” Journal of Social Science Research 3 (5): 8861–8872.
Pangesti, A. D., D. N. A. Susanti, and S. Kusumadewi. 2018. “Perbedaan Efektivitas Obat Kumur yang Mengandung Chlorhexidine dan Essential Oils terhadap Penurunan Tingkat Halitosis.” Bali Dental Journal 2: 49–53.
Paulson, D. R., A. Ingleshwar, N. Theis-Mahon, L. Lin, and M. T. John. 2025. “The Correlation between Oral and General Health-Related Quality of Life in Adults: A Systematic Review and Meta-Analysis.” Journal of Evidence-Based Dental Practice 25 (1): 102078. https://doi.org/10.1016/j.jebdp.2024.102078.
Singh, R., M. A. M. Shushni, and A. Belkheir. 2015. “Antibacterial and Antioxidant Activities of Mentha piperita L.” Arabian Journal of Chemistry 8 (3): 322–328. https://doi.org/10.1016/j.arabjc.2011.01.019.
Sústriková, A., and I. Šalamon. 2018. “Essential Oil of Peppermint (Mentha × piperita L.) from Fields in Eastern Slovakia.” Horticultural Science 31 (1): 31–36. https://doi.org/10.17221/3789-hortsci.
Thapa, P., K. K. Aryal, S. Mehata, A. Vaidya, B. K. Jha, M. Dhimal, S. Pradhan, P. Dhakal, A. Pandit, A. R. Pandey, B. Bista, A. U. Pokhrel, and K. B. Karki. 2016. “Oral Hygiene Practices and Their Socio-Demographic Correlates among Nepalese Adult: Evidence from Non-Communicable Diseases Risk Factors STEPS Survey Nepal 2013.” BMC Oral Health 16 (1). https://doi.org/10.1186/s12903-016-0294-9.
World Health Organization. 2022. Global Oral Health Status Report: Towards Universal Health Coverage for Oral Health by 2030. Geneva: World Health Organization.