Nutmeg Seed Essential Oil Hand Cream: Formulation and In Vitro Antibacterial Activity Against Staphylococcus aureus
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The skin of the palms is easily exposed to chemicals and microorganisms, making it prone to dryness, irritation, and infection, including Staphylococcus aureus. Excessive use of alcohol based hand sanitizers may worsen skin dryness, so an alternative hand cream with antibacterial properties is needed. This study aimed to formulate nutmeg seed essential oil into a hand cream, evaluate its physicochemical characteristics, and assess its in vitro antibacterial activity against S. aureus. This experimental study used essential oil concentrations of 5.0% (F1), 7.5% (F2), and 10.0% (F3); a formulation without oil (F0) was the negative control. Evaluations included organoleptic characteristics, homogeneity, emulsion type, pH, viscosity, spreadability, adhesiveness, freeze-thaw cycling, and hedonic tests. Antibacterial activity was evaluated by agar well diffusion (three technical replicates from one formulation batch) and analyzed using One-Way ANOVA and Tukey HSD. All formulations were homogeneous, semisolid oil-in-water (O/W) emulsions without phase separation after three freeze-thaw cycles. The pH values (6.56–7.64) did not meet the recommended skin pH range of 4.5–6.5, whereas viscosity (17,333.33–34,000.00 cPs), spreadability (6.25–6.90 cm), and adhesiveness met the reference requirements. Mean inhibition zones increased with oil concentration: 4.33 mm (F1, weak), 10.00 mm (F2, moderate), and 10.83 mm (F3, strong), versus 12.00 mm for the positive control (gentamicin 0.1% cream). ANOVA showed a significant difference among groups (F = 35.679; p < 0.001); F2 and F3 did not differ significantly from each other (p = 0.954) or from the positive control (p = 0.498 and 0.864). Nutmeg seed essential oil can be formulated into a hand cream that was physically stable under the freeze-thaw conditions tested and showed in vitro antibacterial activity; F2 (7.5%) was the most efficient concentration. These findings are preliminary: one bacterial strain and one batch were used, and irritation, moisturizing efficacy, and long-term stability were not evaluated.
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Referensi
Ariyadi, R., Maulani, P. A., Ruhimat, U., & Hidana, R. (2023). Identification Of Staphylococcus Aureus Bacteria On The Palms Of Visitors To Panumbangan Health Center. Mukhtabar : Journal Of Medical Laboratory Technology, 1(2), 57–64. Https://Doi.Org/10.52221/Mjmlt.V1i2.376
Ashokkumar, K., & Simal-Gandara, J. (2022). Nutmeg ( Myristica Fragrans Houtt .) Essential Oil : A Review On Its Composition , Biological , And Pharmacological Activities. December 2021, 2839–2851. Https://Doi.Org/10.1002/Ptr.7491
Balouri, M., Sadiki, M., & Ibnsouda, S. K. (2016). Methods For In Vitro Evaluating Antimicrobial Activity: A Review. Journal Of Pharmaceutical Analysisi, 6(2), 71–79. Https://Doi.Org/Https://Doi.Org/10.1016/J.Jpha.2015.11.005
Davis, W. W., & Stout, T. R. (1971). Disc Plate Method Of Microbiological Antibiotic Assay. American Society For Microbiology, 22(4), 659–665. Https://Doi.Org/Https://Doi.Org/10.1128/Am.22.4.659-665.1971
Djarami, J., Pelu, A. D., & Bugis, S. (2020). Formulasi Minyak Gosok Dari Biji Pala (Myristica Fragrans) Dan Uji Antibakteri Terhadap Bakteri Staphylococcus Aureus. Jurnal Fisioterapi Dan Ilmu Kesehatan Sisthana (JUFDIKES). Https://Doi.Org/Https://Doi.Org/10.55606/Jufdikes.V2i1.152
Efendi, Y. N., Sambodo, D. K., & Pratiwi, Dyah Anggraeni Budhi. (2025). Evaluasi Uji Klinik Fase Satu Dan Uji Hedonik Krim Kombinasi Ekstrak Rumput Laut Merah Sumbawa Dan Ekstrak Kulit Buah Jeruk Lemon. 9(1), 40–46. Https://Journal.Stikessuryaglobal.Ac.Id/Index.Php/Hspj/Article/View/1001
Ernawati, E., & Jannah, N. (2021). Aktivitas Antimikroba Perasan Daun Kirinyuh (Chromolaena Odorata L.) Terhadap Candida Albicans Dan Pseudomonas Aeruginosa. Jurnal Kedokteran Dan Kesehatan, 17(2), 137. Https://Doi.Org/10.24853/Jkk.17.2.137-144
Estefania, K. V., Silalahi, J., Sumaiyah, S., & Satria, D. (2022). Formulation And Evaluation Of Cream Turmeric Extract Preparations From Turmeric Rhizomes (Curcuma Domestica Val.). Indonesian Journal Of Pharmaceutical And Clinical Research, 5(1), 01–09. Https://Doi.Org/10.32734/Idjpcr.V5i1.6479
Field, A. (2018). Discoverng Statistics Using IBM SPSS Statistics. SAGE Publications.
Husna, C. A., Hanina, Humaryanto, Gading, P. W., Aurora, W. I. D., & Harahap, H. (2018). Nurul Iman Tentang Infeksi Staphylococcus Aureus Di Kulit Dengan Metode Penyuluhan. AVERROUS: Jurnal Kedokteran Dan Kesehatan Malikussaleh, 5(2), 99. Https://Doi.Org/10.29103/Averrous.V4i2.1041
Kamelia, L. P. L., & Silalahi, P. Y. (2018). BUAH PALA. 11(April), 96–101.
Lachman, Leon, E., Lieberman, Herbert A., E., Kanig, Joseph L., E., & Siti Suyatmi, T. (1994). Teori Dan Praktek Farmasi Industri 3 (H. A. L. Dan J. L. K. Leon Lachman (Ed.); Third Edit). Jakarta: UI-Press, 1994. Https://Lib.Ui.Ac.Id/Detail?Id=137426&Lokasi=Lokal
Loyd V. Allen, Jr., P., Nicholas G. Popovich, P., & Howard C. Ansel, P. (2011). PHARMACEUTICAL DOSAGE FORMS AND DRUG DELIVERY SYSTEMS (D. B. Troy (Ed.); 9th Editio).
Luhulima, A. M. (2021). Formulasi Dan Uji Sifat Fisik Sediaan Krim Ekstrak Etanol Buah Pala ( Myristica Fragrans ) Sebagai Anti Aging. 5(2), 46–59.
Matulyte, I., Jekabsone, A., Jankauskaite, L., Zavistanaviciute, P., Sakiene, V., Bartkiene, E., Ruzauskas, M., Kopustinskiene, D. M., Santini, A., & Bernatoniene, J. (2020). The Essential Oil And Hydrolats From Myristica Fragrans Seeds With Magnesium Aluminometasilicate.
Moningka, M. V., Pareta, D. N., Hariyadi, H., & Potalangi, N. (2020). Formulasi Dan Uji Aktivitas Antibakteri Sediaan Sabun Cair Ekstrak Daun Pala Myristica Fragrans Houtt. Biofarmasetikal Tropis, 3(2), 17–26. Https://Doi.Org/10.55724/J.Biofar.Trop.V3i2.280
Oo, T., Saiboonjan, B., Srijampa, S., Srisrattakarn, A., Suttahanut, K., Chanawong, A., Lulitanond, A., & Tippayawat, P. (2021). Inhibition Of Bacterial Efflux Pumps By Crude Extracts And Essential Oil From Myristica Fragrans Houtt. (Nutmeg) Seeds Against Methicillin-Resistant Staphylococcus Aureus. Molecules. Https://Doi.Org/Https:Doi.Org/10.3390/Molecules26154662
Rahmawati Amalia Yunia. (2020). Disinfektan Surfaktan. July, 1–23.
Sari, F., Angreni, W., Halimah, N., Meutia, R., Sabrina, N. I., Farmasi, D., Kuala, U. S., Aceh, B., Aceh, B., Aceh, B., Farmasi, D., Kuala, U. S., & Aceh, B. (2025). FORMULASI DAN UJI ANTIBAKTERI Staphylococcus Aureus EKSTRAK ETANOL BIJI BUAH PALA ( Myristica Fragrans Houtt ) DALAM SEDIAAN SALEP FORMULATION AND ANTIBACTERIAL TESTING Staphylococcus Aureus ETHANOL EXTRACT OF NUTMEG SEEDS ( Myristica Fragrans Houtt ) Pub. 9(2), 157–163.
Sari, Y. R., & Niken. (2022). Uji Daya Hambat Ekstrak Daun Kirinyuh (Chromolaena Odorata) Terhadap Pertumbuhan Bakteri Staphylococcus Aureus. Jurnal Kesehatan Saintika Meditory, 4(4657), 78–84.
Skhreli, R., Terziu, R., Memushaj, L., & Dhamo, K. (2022). Formulation And Stability Evaluation Of A Cosmetics Emulsion Loaded With Different Concentrations Of Synthetic And Natural Preservative. 5(1). Https://Doi.Org/Https://Doi.Org/10.62400/Jbs.V5i1.6373
SURINI, S., & NEGORO, N. M. (2020). DEVELOPMENT OF MICROEMULSION AND WATER/OIL/WATER MULTIPLE EMULSION CONTAINING BETA-ARBUTIN, LACTIC ACID, AND SODIUM ASCORBYL PHOSPHATE. International Journal Of Applied Pharmaceutics, 212–220. Https://Doi.Org/10.22159/Ijap.2020.V12s1.FF048
Suyasa, I. B. O. (2020). Gambaran Methicillin Resistant Staphylococcus Aureus (MRSA) Pada Petugas Kesehatan RSUD Wangaya Kota Denpasar. Meditory : The Journal Of Medical Laboratory, 8(1), 46–52. Https://Doi.Org/10.33992/M.V8i1.1074
Taylor, T. A., & Unakal, C. G. (2025). Staphylococcus Aureus Infection. In Statpearls. Http://Www.Ncbi.Nlm.Nih.Gov/Pubmed/9709046
Wulandari, S., Wulan, P., Pane, E. B., Adzki, R. A., & Saputra, D. (2023). Perilaku Hidup Bersih Dan Sehat (Tata Cara Pembuatan Hand Sanitizer). 3(1), 38–44.